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Cannabis & Hemp Testing Resources

The twelve-page cannabis brochure and hemp brochure provide a general overview of potency testing, terpene profiling, pesticide analysis, residual solvents, heavy metals and moisture content. From balances to HPLCs and GCMS, Shimadzu has you covered!

The Rise of Cannabinomics

U.S. Medical Marijuana Empowers A
Cannabis Laboratory Revolution (PDF)

The Analytical Scientist

Unraveling the Cannabinome (PDF)

A Closer Look at Cannabis Testing

New to cannabis testing?
Download this whitepaper (PDF)

Basic Principles and Theory of GCMS and LCMS

Shimadzu has a long-standing history of GCMS and LCMS. In these two primers, which serve as textbooks as well as practical application guides, we describe the principles of GCMS and LCMS and provide a better understanding of the innovative technology behind mass spectrometry.

Inflorescence Application Notes

Industrial Hemp Application Notes & Related Information

Articles

Cannabis Industry Journal’s #1 Most Read Article of 2019

From using an HPLC for potency testing to an LC-MS/MS for mycotoxin analysis, find out what instruments you need in a lab to get started.

Shimadzu’s Hemp Analyzer Recognized

Shimadzu is honored to be awarded by independent judges at Cannabis Scientist Magazine for its Hemp Analyzer for cannabinoid analysis, recognized as one of the “Top 5 Tools” that have made cannabis scientists’ lives easier. The judges said.

“I have used both the Hemp Analyzer and Cannabis Analyzer in the field. This instrument revolutionizes potency analysis and is extremely user-friendly.”

“It is important for growers to be able to do testing due to the legal and financial risk involved in growing hemp. Simplifying LC is likely a better approach than some of the other technologies, due to the legal requirements involved. Results can also be correlated with laboratory results.”

Click on the link to learn about other innovations that streamline the cannabis analysis workflow. Use a browser other than Internet Explorer to access Cannabis Scientist Magazine.

Thank you for the recognition

On behalf of Shimadzu, we want to extend our deepest appreciation to our customers and partners for voting Scott Kuzdzal and Robert Clifford to The Cannabis Scientist’s Power List for 2020. Inclusion in this list would not have been possible without your support. This recognition is also a testament to the entire Shimadzu team of dedicated product managers & specialists, tech support scientists, and service engineers, and we thank all team members for their commitment to the cannabis industry.

We will do our very best to continue earning your support. Thank you again!

Validation of Cannabinoids by HPLC-UV

Cannabis has regained much attention as a result of updated legislation authorizing many different uses and can be classified on the basis of the content of tetrahydrocannabinol (THC), a psychotropic substance for which there are legal limitations in many countries. For this purpose, accurate qualitative and quantitative determination is essential. The relationship between THC and cannabidiol (CBD) is also significant as the latter substance is endowed with many specific and non-psychoactive proprieties. For these reasons, it becomes increasingly important and urgent to utilize fast, easy, validated, and harmonized procedures for determination of cannabinoids. The procedure described herein allows rapid determination of 10 cannabinoids from the inflorescences of Cannabis sativa L. by extraction with organic solvents. Separation and subsequent detection are by RP-HPLC-UV. Quantification is performed by an external standard method through the construction of calibration curves using pure standard chromatographic reference compounds.

Measuring Heavy Metal Contaminants in Cannabis and Hemp

Measuring Heavy Metal Contaminants in Cannabis and Hemp offers a comprehensive guide to the entire cannabis industry for measuring elemental contaminants in cannabis and hemp. For testing labs, it
describes fundamental principles and practical capabilities of ICP-MS and other AS techniques for measuring heavy metals in cannabis. For state regulators, it compares maximum contaminant limits of heavy metals with those for federally-regulated pharmaceutical materials. For cultivators and processors it helps them to better understand the many sources of heavy metals in cannabis. And for consumers of medical cannabis, it highlights the importance of choosing cannabis products that are safe to use.

Hemp Testing

Eurofins BioDiagnostics provides the testing tools necessary to bolster production of high-quality hemp products and increase value for your customers.

  • Industry-leading infrastructure & technologies to ensure our results exceed your quality standards
  • Expert staff with decades of method development & analytical experience
  • Capabilities to individually evaluate each product & ensure testing accuracy

Fast Turnaround Time

Scientific Excellence

Client Focused

Our Hemp Testing Services:

Cannabinoid Potency

Eurofins BioDiagnostics quantitates potency content for 16 essential cannabinoids using High Performance Liquid Chromatography. Cannabinoids are chemical compounds produced by cannabis plants and are the subject of many research studies aimed at identifying their therapeutic and pharmacological benefits.

Cannabinoids analysis includes: ∆9-THC, ∆8-THC, THCA-A, THCV, THCVA, CBD, CBDA, CBDV, CBDVA, CBG, CBGA, CBL, CBN, CBNA, CBC, & CBCA

Methods for cannabinoid potency:
• HPLC-UV

Residual Solvent Screening

Hemp oil, the key ingredient to many hemp products, is derived through a solvent extraction method. Hemp extractors commonly use solvents including ethanol, isopropyl, butane, and/or propane that have G.R.A.S status (generally regarded as safe). It is important to screen for residual solvents to ensure consumers understand present constituents in their product.

20 solvents: Acetone, Butane, Ethanol, Propane, etc.

Methods for Residual Solvents Screening: GC-MS

Microbial Contaminants

Eurofins BioDiagnostics experts help customers resolve regulatory compliance, FDA detention, and persistent contamination issues. We validate process and environmental controls, and help develop food safety management programs (HACCP, GMP, SSOP, FSMA, and EMP).

Our state-of-the-art laboratories utilize the latest microbiology testing technologies that provide the robustness and sensitivity needed to rapidly quantify and detect pathogenic, spoilage, and probiotic microorganisms.

With substantial experience across all product categories, we deploy multiple advanced molecular methods for routine microbiology testing, including product screening and in-depth strain identification.
• Aerobic Plate Count
• Yeast & Mold
• Shiga toxin E. Coli
• Salmonella
• Aspergillus species A. fumigatus, A. flavus, A. niger, and A. terreus

Methods for Microbial Contaminants:
• Polymerase Chain Reaction (PCR) techniques to increase detection sensitivity and identify pathogens, toxins, yeasts, and molds

Pesticide Residue Screen

Eurofins BioDiagnsotics has the most comprehensive pesticide scope available with over 800 chemical standard compounds in our inventory. We regularly participate in all major global Proficiency Testing programs for pesticide residues for the most common agricultural commodities in commerce.

We offer a series of large multi-residue screens to provide a more cost-effective option for clients in states with Hemp Research Pilot Programs. Panels include:
• Oregon State Pesticide Panel
• California State Pesticide Panel
• USDA NOP: over 300 different compounds

Methods of Pesticide Residue:
• GC-MS
• HPLC-MS

Heavy Metals

Eurofins BioDiagnostics is the world-leader in trace metal analysis with a comprehensive portfolio of methods dedicated to the detection of trace metals. Our technical expertise and market knowledge in various industries allow our clients to verify compliance with the latest regulations in each country and market, both for raw materials and finished goods.

Our laboratories employ the latest analytical technologies to provide the robustness and sensitivity needed for low-level detection of trace metals, both for identification and quantification purposes.

Primary Trace Metal Elements Detected:
• Heavy Metals Package: Arsenic, Cadmium, Lead, and Mercury

Methods of Heavy Metals Analysis
• ICP-MS (Inductively Coupled Plasma-Mass Spectrometry)

Mycotoxin

Certain molds commonly found on agricultural products have the capacity to form chemical substances called mycotoxins that are harmful when eaten by humans or animals. Mycotoxins are associated with crops that experienced stress in the form of environmental conditions during the growth cycle or poor storage conditions.

Mycotoxins can remain in food & feed long after the fungus that produced them has died; therefore, toxins can be present even when there are no visible signs of mold or fungus. A proactive monitoring program for agricultural commodities should always include testing for mycotoxins to ensure the safety of a product.

Methods of Mycotoxin Analysis:
• Enzyme-Linked Immunosorbent Assay (ELISA)
• High-Performance Liquid Chromatography (HPLC) – Fluorescence detection for analyzing aflatoxins B1 B2 G1 G2, aflatoxin M1, and ochratoxin A are the most commonly used on a variety of foods. The methods are based on AOAC and other industry-approved methods.
• Ultra High-Performance Liquid Chromatography Mass Spectrometry (LC-MSMS) – The most sensitive and selective methods available for the other mycotoxins such as the fumonisins, Vomitoxin, T-2/HT-2, Nivalenol, and Patulin. The methods include rugged extraction, cleanup, and analysis by LC-MSMS.

Eurofins BioDiagnostics labs performing mycotoxin testing are accredited under ISO 17025.

Infrared Analysis

Eurofins BioDiagnostics specializes in creating customized IR solutions through the development and maintenance of on-site, cloud-based NIR/IR systems. QTA® systems utilize a variety of manufactured technologies in combination with our own infrared expertise to provide the most appropriate solution and ensure optimal performance. Whether your spot checking a crop for “hot-spots” or verifying potency of an extract product, NIR/IR systems can provide you a secondary analysis within minutes.

Results in minutes not days, ideal for:
• In-field “hot-spot” testing
• Extract oils and isolates potency verification
• Terpene screening for crop prioritization

Plant Sex

Testing for genetic marker of plant sex will save you time and maximize yield. Eurofins BioDiagnostics uses DNA-based gender determination to provide cultivators early identification of male and female plants.

You no longer need to wait six weeks for growth to visual determine sex; now you can safely send your samples within days of generation and we’ll ID the cannabinoid-rich females which your harvest depends.